DEVELOPMENT OF A PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS COLLOIDAL GOLD DETECTION STRIP

UDC 619:578:636.4

 

Li Laiqing – bachelor, engineer, chairman. Guangzhou Youdi Bio-technology Co., Ltd. (3, Juquan Road, Huangpu District, 510663, Guangzhou, People’s Republic of China). E-mail: lilaiqing191@163.com

Liang Huankun – bachelor, assistant engineer, quality manager. Guangzhou Youdi Bio-technology Co., Ltd. (3, Juquan Road, Huangpu District, 510663, Guangzhou, People’s Republic of China). E-mail: 605712026@qq.com

Zhong Shuhai – bachelor, assistant engineer, R&D supervisor. Guangzhou Youdi Bio-technology Co., Ltd. (3, Juquan Road, Huangpu District, 510663, Guangzhou, People’s Republic of China). E-mail: 1627840617@qq.com

Chen Cuicui – master, engineer, project manager. Guangzhou Youdi Bio-technology Co., Ltd. (3, Juquan Road, Huangpu District, 510663, Guangzhou, People’s Republic of China). E-mail: 285020003@qq.com

Leont’yev Viktor Nikolayevich – PhD (Chemistry), Associate Professor, Head of the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: leontiev@belstu.by Voitau Ihar Vital’evich – DSc (Engineering), Professor, Rector. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: voitov100@mail.ru

DOI: https://doi.org/ 10.52065/2520-2669-2024-277-15

 

Key words: porcine reproductive and respiratory syndrome virus, colloidal gold, card, nucleocapsid N protein..

 

For citation: Li L., Liang H., Zhong S., Chen C., Leont’yev V. N., Voitau I. V. Development of a porcine reproductive and respiratory syndrome virus colloidal gold detection strip. Proceedings of BSTU, issue 2, Chemical Engineering, Biotechnologies, Geoecology, 2024, no. 1 (277), pp. 111–117. DOI: 10.52065/2520-2669-2024-277-15.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important causative agents to the worldwide swine industry, caused significant economic losses annually. Here, we aims to prepare a colloidal gold cards for PRRSV early screening. A double-antibody sandwich colloidal gold cards were optimized and prepared using recombinant PRRSV antigen and its paired monoclonal antibody. The test performance of the colloidal gold cards were evaluated using positive and negative samples. We successfully prepared the colloidal gold cards for fast screening of PRRSV. The colloidal gold cards has no cross reaction with various serum samples, showed high specificity. The detection sensitivity reached 1:800. The colloidal gold cards can be stably stored at 37°C for 7 days without any decrease in sensitivity. At 1:800 dilution, the repeatability was still good. The prepared colloidal gold cards has high specificity, sensitivity, stability and repeatability, it provided an effective monitoring of PRRSV infection.

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09.11.2023