SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF THE PHARMACEUTICAL SUBSTANCE AMBROXOL HYDROCHLORIDE IN AIR

UDC 543.422.3:613.6:661.12

  • Lebedinskaya Kristina Sergeevna – leading laboratory assistant, the Chromatographic Research Laboratory. State enterprise “SPCH” (8, Akademicheskaya str., 220012, Minsk, Republic of Belarus). Е-mail: chromatographic@rspch.by

  • Krymskaya Tatiana Petrovna – Head of the Chromatographic Research Laboratory. State enterprise “SPCH” (8, Akademicheskaya str., 220012, Minsk, Republic of Belarus). Е-mail: chromatographic@rspch.by

  • Lamotkin Sergey Aleksandrovich – PhD (Chemistry), Associate Professor, Head of the Department of Physical-Chemical Methods and Quality Assurance. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: jossby@rambler.ru

DOI: https://doi.org/10.52065/2520-2669-2023-265-1-4

Keywords: ambroxol hydrochloride, mucolytic agent, working air, method of determination, spectrophotometric method.

For citation: Lebedinskaya K. S., Krymskaya T. P., Lamotkin S. A. Spectrophotometric method for the determination of the pharmaceutical substance ambroxol hydrochloride in air. Proceedings of BSTU, issue 2, Chemical Engineering, Biotechnologies, Geoecology, 2023, no. 1 (265), pp. 30–35. DOI: https://doi.org/10.52065/2520-2669-2023-265-1-4 (In Russian).

Abstract

A spectrophotometric technique has been developed for the determination of the mass concentration of ambroxol hydrochloride in the air of pharmaceutical plants and in atmospheric air. This technique is based on the concentration of ambroxol hydrochloride from the air on paper filters AFA-VP followed by its extraction from the filters with methanol under the action of ultrasound, concentration of the extract by complete removal of methanol, dissolution of the dry residue in distilled water, then a derivatisation reaction with 3-methyl-2- benzothiazolinone hydrazone hydrochloride in the presence of cerium (IV) sulphate followed by determination of the crimson coloured reaction product by spectrophotometric method at 570 nm with an absorbing layer length of 1 cm. The following metrological characteristics of the technique for determination of ambroxol hydrochloride in working air were established: repeatability limit r = 11%, intermediate precision limit RI(TO) = 29%, expanded uncertainty U = 23% at confidence probability P = 0.95 and coverage coefficient k = 2.

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