DEVELOPMENT OF A SET OF PRIMERS FOR THE DIAGNOSIS OF OPHIOSTOMA FUNGI ASSOCIATED WITH DRYING PROCESSES OF ENGLISH OAK

UDC 577.212:632.4

  • Baranov Oleg Yurievich − DSc (Biological), Associate Professor, Academician-Secretary of the Department of Biological Sciences. National Academy of Sciences of Belarus (66, Nezavisimosti Ave., 220072, Minsk, Republic of Belarus); Professor, Department of Forest Protection and Wood Science. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: betula-belarus@mail.ru

  • Ivashchenko Lyubov Olegovna − Junior Researcher, Department of Forest Protection and Wood Science. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: lyba281997@mail.ru

DOI: https://doi.org/10.52065/2519-402X-2023-264-05

Keywords: Ophiostoma fungi, Quercus robur, primer, desiccation, PCR.

For citation: Baranov O. Yu., Ivashchenko L. O. Development of a set of primers for the diagnosis of ophiostoma fungi associated with drying processes of English oak. Proceedings of BSTU, issue 1, Forestry. Nature Management. Processing of Renewable Resources, 2023, no. 1 (264), pp. 41–48. DOI: https://doi.org/10.52065/2519-402X-2023-264-05 (In Russian).

Abstract

Polymerase chain reaction (PCR) is a technology based on enzymatic amplification of DNA template in vitro, which is used for rapid detection, characterization and identification of various biological organisms. One of the most important stages in the PCR analysis is the development of primers that allow the most accurate diagnosis of the object under study. This article describes the technology for the development of oligonucleotide primers specific for phytopathogenic fungi of the family Ophiostomataceae Nannf., associated with the lesion of the vascular system of English oak Quercus robur L. The approach is based on the use of genus-specific DNA regions to create oligonucleotide sequences.

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22.10.2022