CHARACTERISTICS OF FILAMENTOUS FORMS OF MICROORGANISMS ISOLATED FROM THE BIOCENOSIS OF ACTIVATED SLUDGE FROM WASTEWATER TREATMENT PLANTS

UDC 628.355.5

 

Masekhnovich Aleksandra Andreevna – PhD student, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: al.masekhnovich@ mail.ru

Grebenchikova Irina Aleksandrovna – PhD (Engineering), Associate Professor, Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: greb_irina_al@mail.ru

Markevich Raisa Mikhaylovna – PhD (Chemistry), Associate Professor, Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: marami-bstu@yandex.ru

Rymovskaya Mariya Vasilʼyevna – PhD (Engineering), Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: rymovskaya_mv@mail.ru

 

DOI: https://doi.org/ 10.52065/2520-2669-2024-283-19.

Key words: wastewater, activated sludge, biocenosis, filamentous forms of microorganisms, bacterial isolates, morphological and biochemical characteristics.

For citation: Masekhnovich A. A., Grebenchikova I. A., Markevich R. M., Rymovskaya M. V. Characteristics of filamentous forms of microorganisms isolated from the biocenosis of activated sludge from wastewater treatment plants. Proceedings of BSTU, issue 2, Chemical Engineering, Biotechnologies, Geoecology, 2024, no. 2 (283), pp. 167–175 (In Russian). DOI: 10.52065/2520-2669-2024-283-19.

Abstract

Currently, more than thirty different types of bacteria are known to cause bulking of activated sludge to one degree or another, but most of them do not have names, but have a number, since their characteristics have not yet been fully studied. The aim of the work was to study the morphological and physiological- biochemical features of filamentous forms of microorganisms isolated from the biocenosis of activated sludge from wastewater treatment plants. Pure cultures of twenty-eight isolates of filamentous forms of microorganisms isolated from biocenoses of urban wastewater treatment plants were obtained. Their morphological and a number of physiological and biochemical characteristics have been determined. It was found that most of the studied bacteria have a rod-shaped shape, are gram-positive, are capable of forming endospores, form filamentous structures with a diameter of 1.2–2.3 μm enclosed in cases. The spectrum of carbon and nitrogen compounds utilized by bacteria has been revealed. It was determined that the preferred substrate for the studied filamentous microorganisms is the carbon of amino acids, the sources of nitrogen are urea and amino acids. Catalase activity was found in most of the isolated bacteria, which indicates their resistance to adverse environmental factors, including the presence of toxicants in the environment. A number of microorganisms demonstrated oxidase activity, which is an indicator of the high rate of oxidation of various substrates by them. It is recommended to use R2A medium to store the studied filamentous forms of bacteria. The data obtained will serve as a basis for the identification of filamentous forms of bacteria and to identify their significance in the development of filamentous bulking of activated sludge in bioreactors of urban wastewater treatment plants.

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References

  1. Markevich R. M., Grebenchikova I. A., Rymovskaya M. V. Biotekhnologicheskaya pererabotka promyshlennykh otkhodov [Biotechnological processing of industrial waste]. Minsk, BGTU Publ., 2018. 300 p. (In Russian).
  2. Yurchenko V. A., Dyagovets Ya. S., Khromenkova E. S., Ostapova A. S. Using microscopy to assess the ecologically significant characteristics of various microbiocenoses. Vestnik KhNADU [Bulletin of the Kharkiv National Automobile and Road University], 2008, no. 43, pp. 92–97 (In Russian).
  3. Reasoner D. J., Geldreich E. E. A new medium for the enumeration and subculture of bacteria from potable water. Applied and Environmental Microbiology, 1985, vol. 49, no. 1, pp. 1–7. DOI: 10.1128/aem.49.1.1-7.1985.
  4. Blackall L. L., Parlett J. H., Hayward A. C., Minnikin D. E., Greenfield P. F., Harbers A. E. Nocardia pinensis sp. nov., an actinomycete found in activated sludge foams in Australia. The Journal of General Microbiology, 1989, vol. 135, no. 6, pp. 1547–1558. DOI: 10.1099/00221287-135-6-1547.
  5. Eikelboom D. H. Filamentous organisms observed in activated sludge. Water Research, 1975, vol. 9, no. 4, pp. 365–388. DOI: 10.1016/0043-1354(75)90182-7.
  6. Stokes J. L. Studies on the filamentous sheathed iron bacterium Sphaerotilus natans. Journal of Bacteriology, 1954, vol. 67, no. 3, pp. 278–291. DOI: 10.1128/jb.67.3.278-291.1954.
  7. Siering P. L., Ghiorse W. C. Phylogeny of the Sphaerotilus-Leptothrix group inferred from morphological comparisons, genomic fingerprinting, and 16S ribosomal DNA sequence analyses. International Journal of Systematic and Evolutionary Microbiology, 1996, vol. 46, no. 1, pp. 173–182. DOI: 10.1099/00207713-46-1-173.
  8. Kuznetsova E. A. Mikrobiologiya [Microbiology]. Orel, OrelGTU Publ., 2005. 188 p. (In Russian).
  9. Belyasova N. A. Mikrobiologiya. Laboratornyy praktikum [Microbiology. Laboratory workshop]. Minsk, BGTU Publ., 2007. 160 p. (In Russian).
  10. Chubenko G. I. Metody identifikatsii bakteriy [Methods of identification of bacteria]. Blagoveshchensk, FGBOU VO Amurskaya GMA Publ., 2018. 44 p. (In Russian).
  11. Gritskevich E. R., Ikonnikova N. V., Buchenkov I. E., Ryshkel’ O. S., Shutova A. G. Laboratornyy praktikum po mikrobiologii [Laboratory workshop on microbiology]. Minsk, IVTs Minfina Publ., 2017. 113 p. (In Russian).
  12. Ivshina I. B., Kamenskikh T. N., Tyumina E. A., El’kin A. A. Mikrobiologiya. Rabochaya tetrad’ dlya laboratorno-prakticheskikh zanyatiy [Microbiology. Workbook for laboratory and practical training]. Perm, PGNIU Publ., 2022. 100 p. (In Russian).
  13. Kontsevaya I. I. Mikrobiologiya: kul’tivirovaniye i rost bakteriy [Microbiology: cultivation and growth of bacteria]. Chernigov, Izdatel’stvo “Desna Poligraf” Publ., 2017. 44 p. (In Russian).
  14. Krasnikova L. V., Gun’kova P. I. Obshchaya i pishchevaya mikrobiologiya [General and Food Microbiology]. St. Petersburg, Universitet ITMO Publ., 2016. 134 p. (In Russian).
  15. Belyasova N. A. Mikrobiologiya [Microbiology]. Minsk, BGTU Publ., 2005. 292 p. (In Russian).
  16. Kämpfer P. Detection and cultivation of filamentous bacteria from activated sludge. FEMS Microbiology Ecology, 1997, vol. 23, no. 3, pp. 169–181. DOI: 10.1111/j.1574-6941.1997.tb00400.x.

13.06.2024