THE INFLUENCE OF CULTIVATION CONDITIONS ON THE MORPHOLOGICAL CHARACTERISTICS OF FILAMENTOUS FORMS OF BACTERIA ISOLATED FROM BIOCENOSES OF WASTEWATER TREATMENT PLANTS

UDC 628.355.5

 

Masekhnovich Aleksandra Andreevna – PhD student, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: al.masekhnovich@ mail.ru

Grebenchikova Irina Aleksandrovna – PhD (Engineering), Associate Professor, Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: greb_irina_al@mail.ru

Markevich Raisa Mikhaylovna – PhD (Chemistry), Associate Professor, Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: marami-bstu@yandex.ru

Rymovskaya Mariya Vasilʼyevna – PhD (Engineering), Assistant Professor, the Department of Biotechnology. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: rymovskaya_mv@mail.ru

 

DOI: https://doi.org/ 10.52065/2520-2669-2024-283-18.

Key words: wastewater, activated sludge, biocenosis, filamentous bacteria, morphological characteristics.

For citation: Masekhnovich A. A., Grebenchikova I. A., Markevich R. M., Rymovskaya M. V. The influence of cultivation conditions on the morphological characteristics of filamentous forms of bacteria isolated from biocenoses of wastewater treatment plants. Proceedings of BSTU, issue 2, Chemical Engineering, Biotechnologies, Geoecology, 2024, no. 2 (283), pp. 161–166 (In Russian). DOI: 10.52065/2520-2669-2024-283-18.

Abstract

Despite the wide variety of methods to combat filamentous bulking of activated sludge, insufficient data has been accumulated to effectively address this problem. Therefore, the search for ways to effectively suppress the development of filamentous forms of microorganisms is an important ecological and biotechnological task. The influence of cultivation conditions on the morphological characteristics of filamentous bacteria isolated from biocenoses of urban wastewater treatment plants has been established. It has been shown that with insufficient aeration or in its absence, most isolates are characterized by the intensive formation of a large number of long filaments, while in aerobic conditions, the degradation of filamentous structures occurs. Cultivation in a nutrient-poor environment promotes the formation of longer filaments regardless of aeration conditions. Prolonged cultivation without adding fresh nutrient medium leads to the breakdown of filaments into individual cells on 5–10 days from the beginning of the experiment in aerobic and on 20–25 days in microaerobic and anaerobic conditions. The mass development of bacteria in a number of isolates can be observed under high loads of organic substances under conditions of intensive aeration, as well as under microaerobic conditions. The most significant changes in the pH values of the culture fluid in comparison with the initial ones were noted in conditions of limited oxygen access to microorganisms. The pH values of the medium in the range of 6–8 contribute to the formation of a large number of filamentous structures by bacteria. A decrease or increase in the pH level leads to degradation of filamentous structures with the formation of medium-length filaments or single cells.

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13.06.2024