IDENTIFICATION OF VARIETIES AND INTRASPECIFIC TAXONS OF LOW BUSH BLUEBERRY (VACCINIUM ANGUSTIFOLIUM AIT.) BASED ON ANALYSIS OF MICROSATELITE LOCUSES (SSR-MARKERS). PROSPECTS FOR THE PRACTICAL USE OF DATA

UDC 631.543.1:634.73:57.06

 

Yukhimuk Andrei Nikolaevich – Reseacher, the Department of the Biochemistry and Biotechnology of Plants. Central Botanical Garden of the National Academy of Sciences of Belarus (2v, Surganova str., 220012, Minsk, Republic of Belarus). E-mail: yukhimuk@cbg.org.by

Gordey Dmitriy Vasil’yevich – PhD (Biology), Senior Lecturer, the Department of Tourism, Nature Management and Game Management. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: gordey@belstu.by

Reshetnikov Vladimir Nikolaevich – Academician, Professor, Head of the Department of the Biochemistry and Biotechnology of Plants. Central Botanical Garden of the National Academy of Sciences of Belarus (2v, Surganova str., 220012, Minsk, Republic of Belarus). E-mail: reshetnikov@cbg.org.by

 

DOI: https://doi.org/ 10.52065/2519-402X-2024-282-17.

 

Keywords: low bush blueberry, variety patent, UPOV, genetic passport, DNA identification, microsatellite loci, DNA markers, marker-assisted selection.

For citation:Yukhimuk A. N., Gordey D. V., Reshetnikov V. N. Identification of varieties and intraspecific taxons of low bush blueberry (Vaccinium angustifolium Ait.) based on analysis of microsatelite locuses (SSR-markers). Prospects for the practical use of data. Proceedings of BSTU, issue 1, Forestry. Nature Management. Processing of Renewable Resources, 2024, no. 2 (282), pp. 141–151 (In Russian). DOI: 10.52065/2519-402X-2024-282-17.

Abstract

DNA identification based on analysis of the allelic composition of eight microsatellite loci made it possible to compile unique genetic profiles for eleven genotypes of blueberry. The highest resolving power was demonstrated by the primers CA421F, Pr031818819a, NA1040 and GVC-C179a, the information polymorphism index of which was 0.80–0.88. The least informative primer was KAN262 with an information polymorphism index of 0.66. Data on the total allelic state of loci and the size of alleles have found practical application to confirm the authenticity of three varieties of low bush blueberry of Belarusian selection. A theoretical assumption has been made about the possibility of using genetic information to assess the effectiveness of crossing hybrids already at the early stages of their ontogenesis by analyzing the combinative variability of a family group or by unique alleles of parental genotypes inherited by the offspring. For the practical use of DNA identification data, immediate improvement of the law in the Republic of Belarus is required, which at the level of regulatory legal acts will consolidate views about “genetic information” and determine the width of the legal field of its use. First of all, we are talking about the approval of a single form of a plant’s genetic passport (formally the result of DNA identification) and the provision of the opportunity to obtain a patent for a variety based on its unique genetic profile.

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