THE DEVELOPMENT OF A METHOD FOR MYCORRHIZAL FUNGI MYCELIA PRODUCING FOR INOCULATION INTO SOIL SUBSTRATES

UDC 579.64:579.69:576.533:604.2:630*181.351

 

Ostrikova Marina Yakovlevna – PhD (Biology), Senior Researcher, the Research Department of Genetics, Breeding and Biotechnology. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246050, Gomel, Republic of Belarus). E-mail: heterobasidion@mail.ru

Razumova Olga Aleksandrovna – PhD (Biology), Leading Researcher, the Laboratory of Genomic Research and Bioinformatics. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246050, Gomel, Republic of Belarus). E-mail: kovalevich@mail.ru

Konstantinov Andrey Vyacheslavovich – Master of Biology, Researcher, the Laboratory of Genomic Research and Bioinformatics. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246050, Gomel, Republic of Belarus). E-mail: avkonstantinof@mail.ru

Panteleev Stanislav Viktorovich – PhD (Biology), Associate Professor, Head of the Laboratory of Genomic Research and Bioinformatics. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246050, Gomel, Republic of Belarus). E-mail: stasikdesu@mail.ru

Kharkhasova Irina Aleksееvna – РhD student, the Laboratory of Genomic Research and Bioinformatics. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246050, Gomel, Republic of Belarus). E-mail: harhasova18@mail.ru

 

DOI: https://doi.org/ 10.52065/2519-402X-2024-282-11.

 

Key words: mycorrhizal fungi, pure cultures, in vitro cultivation, mycelium production, substrates.

For citation: Ostrikova M. Ya., Razumova O. A., Konstantinov A. V., Panteleev S. V., Kharkhasova I. A. The development of a method for mycorrhizal fungi mycelia producing for inoculation into soil substrates. Proceedings of BSTU, issue 1, Forestry. Nature Management. Processing of Renewable Resources, 2024, no. 2 (282), pp. 88–94 (In Russian). DOI: 10.52065/2519-402X-2024-282-11.

Abstract

Morphological and cultural characteristics of mycorrhizal fungi representatives during growing on nutrient media of various compositions and organic carriers were studied. Nutrient media ensuring high growth activity and viability of the mycelium during periodic in vitro cultivation were selected. Based on macro- and microsalts according to the MS recipe, supplemented with 10% wort, a mixed composition medium has been developed, which is universal for cultivating the studied species of fungi in order to produce their biological material. The range of variability in morphological and cultural characteristics of various species of mycorrhizal fungi pure cultures was determined depending on the composition of the medium used for cultivation and the biological characteristics of the strains. The possibility of solidphase cultivation of mycelial cultures on organic substrates has been studied. It has been shown that cultivation on sterile leaf litter or its mixture with high-moor peat allows the production of mycelium of target strains. Leaf litter has been identified as the most suitable substrate for mycelium of almost all tested mycorrhizal fungi obtaining. The methods for biomass of promising mycorrhizal fungi obtaining have been developed. This biological material will be used in nurseries as an addition to soil substrates in order to activate mycorrhization process in forest tree seedlings that require it.

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15.03.2024