CHROMATOGRAPHIC ANALYSIS OF HUMAN SWEAT-FAT TRACES

UDC 543.544

  • San’ko Aleksandra Gennad’yevna − Master’s degree student. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: alyaevtuh@mail.ru

  • Stasevich Ol’ga Viktorovna − PhD (Chemistry), Associate Professor, Assistant Professor, the Department of Physical-Chemical Methods for Products Certification. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: stasevich@belstu.by

DOI: https://doi.org/10.52065/2520-2669-2022-259-2-191-194

Key words: sweat-fat traces, thin-layer chromatography, gas-liquid chromatography, mobile phase, identification, fatty acid composition, oleic acid, stearic acid.

For citation: San’ko A. G., Stasevich O. V. Chromatographic analysis of human sweat-fat traces. Proceedings of BSTU, issue 2, Chemical Engineering, Biotechnologies, Geoecology, 2022, no. 2 (259), pp. 191–194 (In Russian). DOI: https://doi.org/10.52065/2520-2669-2022-259-2-191-194.

Abstract

In this work, the analysis of sweat-fat traces of human (female) hands by chromatographic methods was carried out to control changes in its composition over time. The optimal conditions for thin layer chromatography (TLC) were selected, as a result, the most optimal mobile phase was a mixture of petroleum ether – diethyl ether – acetic acid (90 : 10 : 1), when using it on TLC plates, 4 spots with Rf = 0.89 – cholesterol esters, Rf = 0.76 – triacylglycerides, Rf = 0.30 – fatty acids, Rf = 0.13 – cholesterol were identified. Within 4 months this composition remained unchanged. Also sweat-fat traces were analyzed by fatty acid composition by gas-liquid chromategraphy (GLC), which showed the presence in its composition of homologous series of saturated and unsaturated (with varying degrees of unsaturation) fatty acids with the number of carbon atoms in the chain from 4 to 18. In addition, alkanes and aromatic carbohydrates were registered in insignificant amounts, which is in accordance with the literature data. The largest amount was accounted for by fatty acids containing C18 carbon atoms. Most of all, linoleic (9.73%) and linolenic (3.39%) acids were found. It was also established the ratio of the contents of oleic and stearic acids, equal to 0.26, which confirms that the sweat-fat traces belong to a woman.

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16.06.2022