PROCESSES OF HYBRIDIZATION IN SESSILE AND PEDUNCULATE OAK BY THE RESULTS OF MOLECULAR GENETIC ANALYSIS

UDC 630*165.3:630*17:582.632.2

  • Padutov Vladimir Evgen’yevich − DSc (Biology), Corresponding Member of the National Academy of Sciences of Belarus, Head of the Research Department of Genetics, Breeding and Biotechnology. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., Gomel’, 246001, Belarus). E-mail: forestgen@mail.ru

DOI: https://doi.org/10.52065/2519-402X-2021-246-12-93–102

Key words: half-sib families, interspecifc oak hybrids, microsatellites, simple sequence repeats(SSRs), randomly amplifed polymorphic DNA (RAPD).

For citation: Padutov V. E. Processes of hybridization in sessile and pedunculate oak by the results of molecular genetics analysis. Proceedings of BSTU, issue 1, Forestry. Nature Management. Processing of Renewable Resources, 2021, no. 2 (246), pp. 93–102 (In Russian). DOI: https://doi.org/10.52065/2519-402X-2021-246-12-93–102.

Abstract

Molecular genetic analysis of hybrids of pedunculate oak (Quercus robur L.) and sessile oak (Q. petraea [Matt.] Liebl.), as well as offspring from crossing of hybrids and parental species was carried out. All individuals were divided into six following groups reflecting the taxonomic affiliation of plants (based on cross-breeding schemes and studies of morphological characters). They are 1 – Q. petraea (P: Q. petraea); 2 – Q. robur (P: Q. robur); 3 – Hybrid (P: Q. petraea); 4 – Hybrid (P: Q. robur); 5 – Q. petraea (P: Hybrid); 6 – Hybrid (P: Hybrid). The level of genetic polymorphism in each of the six groups and the degree of differentiation between them were assessed using RAPD markers (OP-R11, OPR05, OP-R10, OP-G06) and SSR markers (ssrQpZAG15, ssrQpZAG9, ssrQpZAG46, ssrQpZAG7). Deviations from the equilibrium state of population genetic parameters were revealed, which allow us to speak about assortative mating between the two species. It is shown that a limited number of genotype variants are involved in the introgression process. Lower values of the variability indices and intrapopulation subdivision in genetic structure of hybrid offspring in comparison with the stands of the parental species reflect this tendency.

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