MOLECULAR GENETIC DIAGNOSTICS OF INA BACTERIA IN SCOTS PINE SEEDLINGS WITH INFECTIOUS LODGING

UDC 577.212:632.4

Ivashchenko Lyubov Olegovna – Junior Researcher, the Department of Forest Protection and Wood Science. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus); external doctorate student in the specialty “Forest crops, selection and seed production”. Forest Institute of the National Academy of Sciences of Belarus (71, Proletarskaya str., 246001, Gomel, Republic of Belarus). E-mail: lyba281997@mail.ru

 Baranov Oleg Yurievich – DSc (Biological), Associate Professor, Academician-Secretary, the Department of Biological Sciences. National Academy of Sciences of Belarus (66, Nezavisimosti Ave., 220072, Minsk, Republic of Belarus); Professor, the Department of Forest Protection and Wood Science. Belarusian State Technological University (13a, Sverdlova str., 220006, Minsk, Republic of Belarus). E-mail: betula-belarus@mail.ru

DOI: https://doi.org/ 10.52065/2519-402X-2024-276-10

Key words: ice nucleation bacteria, infectious lodging, ina gene, ice formation, Pseudomonas syringae.

For citation: Ivashchenko L. O., Baranov O. Yu. Molecular genetic diagnostics of INA bacteria in Scots pine seedlings with infectious lodging. Proceedings of BSTU, issue 1, Forestry. Nature Management. Processing of Renewable Resources, 2024, no. 1 (276), pp. 82–86 (In Russian). DOI: 10.52065/2519-402X-2024-276-10.

Abstract

Bacteria that actively form ice nuclei, or ice nucleating bacteria (INA), are both epiphytic and endophytic inhabitants, causing frost damage to many plants at low positive temperatures. To date, information about their participation in the development of pathophysiological processes in plants is scattered and incomplete. One of the important stages in determining the competitiveness of INA bacteria is their identification in plant samples. This article presents the results of determining the information content of typical primers capable of diagnosing ice-nucleating bacteria both in generations of the current environment and on the surface and inside agricultural and forest plants based on amplification of the gene ina encoding the ice formation protein (INP). Conducted studies using plant samples in the form of Scots pine seedlings, features of the components of the infectious lodging, the presence of the gene ina the experimental material.

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10.10.2023